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Species of fish offered to consumers are frequently infested with parasites. Our general aim is therefore to assess i) the prevalence of 3 groups of zoonotic parasites which are the metazoans of the Aniskasis group (Anisakis sp. Contracaecum sp., Pseudoterranova sp., Hysterothylacium sp.) and the protists Cryptosporidium sp. and Blastocystis sp. in seafood products from Boulogne-sur-Mer and ii) the impact of these parasites on the health of workers in the fishing industry and on the general public.
First, we will target the detection and the identification of these organisms in three species of fish (herring, whiting, mackerel), a species of cephalopod (squid) and in marine mammals in collaboration with the ‘Centre de Recherches sur les Mammifères Marins’ (Centre for Research in Marine Mammology) which coordinates the ‘Réseau National d’Echouage’ (National Stranding Network). The parasites will be identified by microscopy (immunofluorescence, specific staining) and/or by molecular biology (PCR and high throughput sequencing) before genotyping.
Then we will carry out a clinical - epidemiological  study whose objective is to compare the frequency of allergies to  Aniskasis or fish antigens in the general public and in professionals from the Boulogne-sur-Mer fishing industry. We will also assess the  respective role of sensitisations to the Aniskasis and/or fish antigens in the triggering of allergic reaction when in contact with fish. These studies will be carried out in collaboration with various departments from Lille Regional Hospital (Immunology, Occupational Medicine, Parasitology-Medical Mycology, Clinical Investigation Centre) and two medical departments from Boulogne-sur-Mer (Occupational Health, Seamen’s Occupational Medicine). These studies will be validated by the Persons Protection Committee.

If the results of the WP2 (clinical – epidemiological part of the project) indicates an increased risk of developing allergic reactions when in contact with fish, an improvement in the working conditions of fishing industry workers will be considered in partnership with businesses: recycling of air in the workshops, gloves, etc. The project will therefore contribute to better allergic risk management.

 - Institut Pasteur: Biologie et Diversité des Pathogènes Eucaryotes Emergents (BDPEE),
 - ANSES Boulogne-sur-Mer,
 - CHRU Lille: Faculty of Medicine Parasitology-Mycology Laboratory,
 - Occupational Health Department,
 - Seaman’s Occupational Health Department,
 - CHRU Lille: Occupational Health and Occupational Parasitology,
 - CHRU Lille: Center for Clinical Investigation,
 - CHRU Lille: Immunology Laboratory,
 - Parasitology-Mycology Laboratory Hôpital Cochin, APHP, Univ. Paris Descartes.

This project was labellised by the AQUIMER Pole of Competitiveness on the 19th April 2013.


16 rue du Commandant Charcot
62200 Boulogne-sur-Mer F

Tel : 00 333 21 10 78 98

Fax : +33 3 21 30 33 22



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